Molecular Pathology

Research into the molecular basis of diseases and pathogen–host interactions in plants. Investigation of the molecular basis of human diseases and the impact of changes at the DNA level on the development and course of a given disease. Microbiome analyses in relation to human health, agriculture, and industrial processes.

Assoc. Prof. Mgr. Jan Lochman, Ph.D.

Group Leader

Assoc. Prof. Lochman received his Ph.D. in Biochemistry from Masaryk University in Brno in 2006. In 2005 and 2006, he spent several scientifically productive months in INRAe laboratories in France, and since 2007 he has worked at the Faculty of Science. He initially worked as an assistant professor; since 2017, he has served as an associate professor. Currently, he is the director of the department and guarantor of the Biochemistry Bachelor's and Master's degree programmes. 

MUNI | orcID | ResearchGate

About Assoc. Prof. Lochman

Contact information:

  • Office – C05/331
  • Phone – 549 49 5602
  • E-mail – lochik@mail.muni.cz

Teaching

  • C7860 Plant Biochemistry
  • C9320 Methods in Biotechnology
  • C7878 Gene Technology
  • C7876 Gene Technology – Practical Training
  • C7175 DNA Diagnostics
  • C7195 Advanced Practical Course in Biochemistry
  • C1200 Introduction to Biochemistry

Education

  • 2017 – Associate Professor, Biochemistry, Faculty of Science, MU, "Molecular Aspects of MAMP Triggered Immunity in Plants"
  • 2006 – Ph.D., Biochemistry, Faculty of Science, MU, "Molecular-Biological Markers of Plant-Pathogen Interaction"
  • 2002 – Mgr., Biochemistry, Faculty of Science, MU

University Activities

  • Director of the Department of Biochemistry
  • Guarantor of the Bachelor’s and Master’s degree programmes in Biochemistry
  • Chair of the Joint Programme Board for Biochemistry
  • Member of the Doctoral Board for Biochemistry and Bioanalytical Chemistry
  • Member of the Academic Senate of Masaryk University
  • Member of the Economic Committee of the Academic Senate of the Faculty of Science, MU

Research Topic

Our primary research objectives focus on elucidating the molecular mechanisms of plant–microorganism interactions and on methods for analysing microbiota composition in human and environmental samples.

Fig. 1 | Analysis of plant defence responses to pathogens and the effect of priming on plant resistance (A), and transcriptomic and metagenomic analyses of a broad range of samples – the complete human microbiome, soils, wastewater treatment plants, rumens, and others (B)

In our study of plant–microorganism interactions, we investigate the mechanisms involved in pathogen recognition and the activation of plant immune responses, with the aim of reducing pesticide and fertiliser use in the global context of sustainable agriculture. In our current research, we systematically focus on a model system involving the interaction of oomycetes and the bacterium Pseudomonas syringae with tobacco, tomato, and Arabidopsis plants. We also integrate findings from molecular pathophysiology and biochemistry to better understand the molecular basis of the phenomenon known as defence priming as a new strategy for protecting crops against biotic and abiotic stress conditions.

Recently, we have also begun studying microbiota from various environments. Using state-of-the-art approaches and instrumentation, we determine not only which bacterial species are present in a given environment (metagenomics), but also investigate their functional activity as determined by gene expression (transcriptomics and metatranscriptomics) and subsequent metabolite production (metabolomics). Combining these approaches allows us to better understand microbiota–environment interactions, which play a key role in a wide range of environmental and pathological processes.

Projects

  • Study of mechanisms involved in the activation of plant immune responses
  • Elucidation of the mechanism of BABA-induced resistance in tomato plants
  • Effect of epigenetic changes on the ability to induce resistance in plants
  • Development of a method for microbiota analysis and monitoring of its functional activity
A scientist in a lab coat and gloves using a syringe to extract a sample from a large bioreactor in a laboratory.

Group Members

Martina Zapletalová, Ph.D. Researcher II – MUNI | orcID | RG

Ph.D. students: David Novák, Veronika Hroudná, Vojtěch Rejtar
Master’s students: Alexandra Farkačová, Ema Galeová, Amálie Halaxová, Nashwan Tahir Salih
Bachelor’s students: Jakub Staňo
Laboratory technician: Bc. Lenka Kociánová
Ph.D. graduates:  Michal Obořil, Tomáš Starý, Hana Uhlíková, Laura Ambrozová, Veronika Juráková, Adam Janotík

 

International Collaboration

No description

Methods

  • Basic molecular biology techniques
  • NGS library preparation (FragmentAnalyzer)
  • Sequencing and fragment analysis (NGS MiniSeq, GeneticAnalyzer 3100)
  • qPCR analysis (LightCycler 480 II, Biometra qPCR system)
  • Heterologous protein expression (E. coli, Pichia pastoris)
  • Measurement of plant resistance (tobacco, tomato, Arabidopsis) to pathogens (H. parasitica, P. parasitica, P. syringae)
  • Proteome and metabolome analysis (Agilent HPLC)

Recent Publications

A list of all publications is available on the MUNI website.

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